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KMID : 0385219960060010058
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Korean Journal of Gerontology
1996 Volume.6 No. 1 p.58 ~ p.65
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Inhibition of Nitric Oxide Generation from Macrophages by Sodium Salicylate
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Chang Hyun-Jung
Lee Eun-A
Shin Jung-Hee
Baek Kwang-Jin
Kwon Nyoun-Soo
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Abstract
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Salicylates including sodium salicylate and acetyl salicylate (Aspirin) are known to inhibit the synthesis of prostaglandins, and frequently used as antiinflammatory agents. In the present study, effect of salicylates on macrophages nitric oxide (NO) generation was examined. NO has been known to play important roles in antimicrobial and antitumor activities of macrophages, and in cytotoxic effects in inflammation. When macrophages-like RAW 264.7 cells were treated with varying concentrations of sodium salicylate ranging from 0.3% to 40 mM, and followed by stimulation with lipoplysaccharide and interferon-Υ, the NO generation was inhibited by a dose-dependent manner. However, acetyl salicylate at the concentrations of 5 mM did not block the NO generation. The inhibitory effect of sodium salicylate on NO generation far exceeded its cytotoxic effect, suggesting that it acts on macrophages to reduce the NO synthesis. NO generation activity of macrophages lysate was markedly inhibited by 10 or 20 mM sodium salicylate. When RAW 264.7 macrophages-like cells and murine peritoneal macrophages were pretreated with sodium salicylate before activating with lipoplysaccharide and interferon-Υ, and washed with fresh medium after the activation, NO generation was still inhabitable. Furthermore, immunoblotting analyses showed that sodium salicylate reduced the expression of NO synthase protein. These results suggested that sodium salicylate inhibits NO generation from macrophages by direct inhibitory effect on NO synthase activity and by decreasing the expression of NO synthase protein.
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KEYWORD
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Nitric oxide, sodium salycilate, macrophage
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